Bicycling is a form of active transportation with a number of health benefits but carries a high risk of injury compared to other transportation modes. Safety intervention evaluations often produce results in the form of ratios, which can be difficult to communicate to policy-makers. The primary objective of this study was to estimate the number of bicycling injuries on an urban corridor preventable by separated bicycling infrastructure. Stakeholders identified a key corridor with multiple segments having bicycling infrastructure but most of the corridor lacking similar infrastructure. We counted bicyclist volume along this route and used secondary data to supplement counts missing due to COVID-19. We used two reference studies including local bicycling population to estimate benefit of separated bicycling infrastructure and applied this to a city-wide estimate of baseline risk of injury per kilometre bicycled, which used a combination of secondary data sources including police, health care and travel survey data. Finally, we adjusted baseline risk to account for increased bicyclist volume during and following the COVID-19 pandemic. We estimated installation of fully separated cycle tracks along one Toronto corridor would prevent approximately 152.9 injuries and 0.9 fatalities over a 10-year period. Our results underscore the benefits of separated bicycling infrastructure. We identify several caveats for our results, including the limitations of studies used to estimate relative risk of infrastructure. Our method could be adapted for use in other cities or along other corridors. Finally, we discuss the role of preventable burden estimates as a knowledge translation tool.